Journal: Molecules

Article Title: Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological Malignancies

doi: 10.3390/molecules28041897

Figure Lengend Snippet: NAMPT inhibitors induce cell death in various cell lines of hematological malignancies. IC 50 (nM) after 96 h of treatment with NAMPT inhibitors.

Article Snippet: Four hematological cell lines (ML2—acute myeloid leukemia; Jurkat—acute lymphoblastic leukemia; Namalwa—Burkitt lymphoma; and RPMI8226—multiple myeloma) were purchased from DSMZ (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) or ATCC.

Techniques:

Journal: Molecules

Article Title: Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological Malignancies

doi: 10.3390/molecules28041897

Figure Lengend Snippet: NAMPT inhibitors induce cell death in hematological malignancies in a dose-dependent manner, with FEI199 being the most potent. A dose-dependent analysis of cell death induced by NAMPT inhibitors on ML2 ( A ), Jurkat ( B ), Namalwa ( C ) and RPMI8226 ( D ) cell lines. The cells were treated with various concentrations of NAMPT inhibitors for 96 h and cell death was assessed by flow cytometry using ANXN and 7AAD staining. The percentages of early apoptotic cells (ANXN + 7AAD − ) are shown as white columns and those of late apoptotic cells (7AAD + ) are shown as solid black columns. Data are means ± SD, n ≥ 3.

Article Snippet: Four hematological cell lines (ML2—acute myeloid leukemia; Jurkat—acute lymphoblastic leukemia; Namalwa—Burkitt lymphoma; and RPMI8226—multiple myeloma) were purchased from DSMZ (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) or ATCC.

Techniques: Flow Cytometry, Staining

Journal: Molecules

Article Title: Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological Malignancies

doi: 10.3390/molecules28041897

Figure Lengend Snippet: NAMPT inhibitors induce necrotic cell death and decrease cell proliferation in hematological malignancies. A time-dependent analysis of LDH release on ML2 ( A ) and Jurkat ( B ) cells treated with NAMPT inhibitors (10 nM) was assessed according to the manufacturer’s instructions. Cell proliferation was assessed on ML2 ( C ) and Jurkat ( D ) cells based on the ability of metabolically active cells to reduce resazurin sodium salt to a highly fluorescence product using AlamarBlue reagent. The absorbance of both products was measured with a spectrophotometer at the appropriate wavelength. Data are ± SD, n = 3, * p < 0.05, ** p < 0.01, **** p < 0.0001 (CTRL vs. inhibitors treated groups).

Article Snippet: Four hematological cell lines (ML2—acute myeloid leukemia; Jurkat—acute lymphoblastic leukemia; Namalwa—Burkitt lymphoma; and RPMI8226—multiple myeloma) were purchased from DSMZ (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) or ATCC.

Techniques: Metabolic Labelling, Fluorescence, Spectrophotometry